ANTIDIABETIC POTENTIALS OF POLYHERBAL (Citrullus colocynthis, Curculigo pilosa, and Gladiolus pssitacinus) EXTRACT IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

Abstract

The art of preparing concoction/decoction using collection of medicinal plants is very common for treatment of ailment in folk medicine. The study explored the antidiabetic potentials of polyherbal extract (PHE) prepared from Citrullus colocynthis, Curculigo pilosa and Gladiolus pssitacinus. Phytochemical profiling, in vitro antioxidant and antidiabetic properties of the PHE were evaluated. Diabetes mellitus (DM) was induced by (i.p) administration of streptozotocin (50 mg/kg). Fasting blood glucose (FBG) and body weight (BW) were monitored in diabetic rats during administration of PHE for 28 days. Effects of treatment on lipid profiles and different markers of liver and kidney functions were determined. Also concentrations of lipid peroxides (LPO), nitric oxide (NO), glutathione (GSH) and activity of superoxide dismutase (SOD) were determined in kidney and liver tissues. The results showed that PHE has phenolic (90.64 mg/100g GAE) and flavonoids (63.97 mg/100g QUE) contents. It also exhibited in vitro antioxidant and inhibitions of amylase and glucosidase. Treatment of diabetic rats with PHE reduced significantly (p<0.05) their FBG. Lipid profiles and serum level of liver and kidney function markers were positively modulated in treated diabetic rats. Levels of LPO and NO were decreased with oral administration of PHE while GSH and activity of SOD were increased. This study has shown that poly- herbal extract possesses antidiabetic potential thereby justifying it usage in traditional medicine for management of DM.